[JACS] 我室杨朝勇教授发表论文:A Synthetic Light-Driven Substrate Channeling System for Precise Regulation of Enzyme Cascade Activity Based on DNA Origami

我室杨朝勇教授在 JACS 上发表论文:A Synthetic Light-Driven Substrate Channeling System for Precise Regulation of Enzyme Cascade Activity Based on DNA Origami

文章链接:https://pubs.acs.org/doi/10.1021/jacs.8b05429

摘要:

Substrate channeling, in which metabolic intermediate is directly passed from one enzyme to the next enzyme in an enzyme cascade, accelerates the processing of metabolites and improves substrate selectivity. Synthetic design and precise control of channeling outside the cellular environment are of significance in areas such as synthetic biology, synthetic chemistry, and biomedicine. In particular, the precise control of synthetic substrate channeling in response to light is highly important, but remains a major challenge. Herein, we develop a photo-responsive molecule-based synthetic substrate channeling system on DNA origami to regulate enzyme cascade activity. The photo-responsive azobenzene molecules introduced into DNA strands enables reversible switch the position of substrate channeling to selectively activate or inhibit the enzyme cascade activity. Moreover, DNA origami allows precise control of inter-enzyme distance and swinging range of the swing arm to optimize the regulation efficiency. By combining the accurate and addressable assembly ability of DNA origami and the clean, rapid and reversible regulation of photo-responsive molecules, this light-driven substrate channeling system is expected to find important applications in synthetic biology and biomedicine.